WebPolyclonal Antibody for studying Beclin 1. Cited in 728 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously validated in-house, Beclin-1 Antibody (CST #3738) is ready … WebApr 1, 2024 · After electrophoresis and membrane transfer, incubation with primary antibodies against Mst1 (3682 S; CST), phospho-Mst1 (Thr183)/MST2 (Thr180) (49,332 S; CST), Beclin-1 (3738 S; CST), p62 antibody (23,214 S; Cell Signalling), LC3-I and LC3-II antibody (2775 S; Cell Signalling) was performed. ... (Thr180) (49,332 S; CST), Beclin-1 …
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WebMay 10, 2024 · RhoB binds with Beclin 1 through its residues at 118 to 140 and the Beclin 1 CCD domain, with RhoB Arg133 being the key binding residue. Binding of RhoB to Beclin 1 enhances the Hsp90-Beclin 1 interaction, preventing Beclin 1 degradation. RhoB also directly interacts with Hsp90, maintaining RhoB levels. WebSep 2, 2024 · The results demonstrated that both Beclin 1 and Atg5 expression were higher in the p27 KD H9c2 cells compared with WT cells after 3 h of hypoxia/ischemia, but attenuated after 12 h (Fig. 3A and B). The ratio of LC3-II/I, a hallmark of autophagosomes, coincided with the expression of Beclin 1 and Atg5 ( Fig. 3B ). grand rapids condos at fitzgerald
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WebApr 1, 2024 · Beclin 1 promotes the lipidation of microtubule-associated protein 1 light chain 3 (LC3-I) to produce the autophagosome-associated form LC3-II during the process of elongation. ATG7 is required for the conjugation of ATG12 to ATG5 and activates LC3-I, which is directly associated with autophagosome formation. WebMammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and … WebBeclin-1 (the mammalian ortholog of yeast ATG6) has been well-characterized to play a pivotal role in autophagy that is a major catabolic pathway in which the cell degrades … grand rapids community mental health services