Ppic9 plasmid
WebpPIC9 10 µg, 20 µL at 0.5 µg/µL in TE buffer, pH 8.0 : Vector for secreted expression in Pichia. Uses the α-factor signal sequence. * ... The AOX1 gene has been isolated and a … WebpPIC9. Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein. Sequence Author: Thermo Fisher ... Home Resources Plasmid Files Yeast Plasmids …
Ppic9 plasmid
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Web86. Buy from Supplier. Pichia Pastoris Expression Vector Ppic9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO … WebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of …
WebUse XhoI and EcoRI to digest PETase-GCW21/51/61 and pPIC9 at 37 °C. 4. Ligate the digested fragments PETase-GCW21/51/61 along with plasmid pPIC9 digested with XhoI and EcoRI at 22 °C for 30 min and transform into E. coli DH5α. Select several single clonies and culture it in LB medium at 37 °C for 12 h, and extract the plasmids. 5. WebCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. …
WebSep 4, 2024 · To construct anti-tumour recombinant polypeptide and evaluate its anti-tumor effects. Anti-tumour recombinant polypeptid was designed based on the gene sequence … Webwas inserted into the pPIC9 K expression vector to form the recombinant plasmid pPIC9 K-FtTI. Expression of the recombinant protein The gene was digested with NotI and EcoRI restriction enzymes, and the generated fragment was ligated into plasmid pPIC9 K. The resulting plasmid (pPIC9 K-FtTI), linearized with SacI restriction enzyme, was used in the
WebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of …
WebDec 1, 2003 · The plasmid pPIC9-NotI minus was digested with EcoRI and AvrII, and ligated to the above PCR fragment treated with EcoRI and AvrII to generate plasmid pPIC9-Fc. The ScFv fragment, screened from a phage antibody library (constructed by Qian Feng, unpublished), can bind Glutathione-S-transferase (GST) by PCR amplification using primer … hometown buffet south portland maineWebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that … his glory david scarlettWebUse XhoI and EcoRI to digest PETase-GCW21/51/61 and pPIC9 at 37 °C. 4. Ligate the digested fragments PETase-GCW21/51/61 along with plasmid pPIC9 digested with XhoI … his glory home pageWebPlasmid Preparation Invitrogen offers a number of plasmid DNA purifications systems. For more information, refer to www.invitrogen.com or contact Technical Support (page 33) Item Amount Cat. no. PureLink™ HiPure Plasmid Miniprep Kit 25 preps 100 preps K2100–02 K2100–03 PureLink™ HiPure Plasmid Midiprep Kit 25 preps 50 preps K2100–04 hometown buffet specials friday nightWebvector pPIC9 or pPIC9K using the DNA ligation kit (TAKARA BIO), and the ligation mixture was applied to transform the E. coli DH5 strain. The constructed plasmids (pPIC9/hsTf and pPIC9K/hsTf), purified from the transformants, were confirmed as such using a DNA sequencer. Transformation of P. pastoris. Transformation of P. pastoris was hometown buffet sunday dinner menuWebPlasmid Preparation Invitrogen offers a number of plasmid DNA purifications systems. For more information, refer to www.invitrogen.com or contact Technical Support (page 33) … hometown buffet store hoursWebJun 9, 2024 · Strains, plasmids, and antibodies. The Pichia pastoris strain GS115, Escherichia coli strain DH5α, and expression vectors pPIC9 and pPICZα were purchased from Invitrogen (Carlsbad, CA). The template plasmids pPIC9-SuIL-15Rα and pPICZα-IgG4-Fc (with mutations S228P, L235E, and N297Q) were constructed and preserved in our … his glory david scarlett drops