WebFeb 12, 2024 · Cell culture and transfection. Human neuroblastoma SH-SY5Y cells were maintained in a minimal essential medium. HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and an antibiotic–antimycotic mix (all from Invitrogen). Cells were grown in a humidified incubator at 37°C with 5% CO 2. WebIMR-32 and SH-SY5Y cells were separately plated in 6-well plates and were transiently transfected with IGF1R or negative control shRNA, according to the manufacturer’s introduction, using RNAi-Mate transfection reagent (GenePharma, China). After 48 h transfection, the cells were applied in the indicated experiments.
Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
WebStable transfection was performed using the XtremeGENE reagent for 72 hours, and 400 µg/ml G418 antibiotics used... SH-SY5Y cells were transfected with a pcDNA3.1 vector plasmid. Stable transfection was performed using the XtremeGENE reagent for 72 hours, and 400 µg/ml G418 antibiotics used... Features; Plans. Academia. Industry. WebNov 14, 2024 · Here, the transfection with miR-15b mimics significantly reduced the reporter activity of NF-κB1 and IKK-α (Figure 6A,D). As reported, putative binding sites for miR-15b were identified in the 3′UTRs of NFKB1 and IKK-α ( Figure 6 C,F). miR-15b failed to regulate either NF-κB1 or IKK-α transcription when the mutation within the corresponding 3′UTRs … curly hair short sides female
RNAiMAX Reverse Transfections Lipofectamine Thermo Fisher Scientific …
WebExcels in cell culture techniques and optimization in E. coli, HEK293, CHO, HeLa, SKNDZ, and SHSY5Y cell lines. Experienced in genetic editing using ZFNs and transfection for high titer yields. Web1.1 Preface. A detailed report by Biedler et al. in 1978 describes the original culturing conditions for SH-SY5Y cells [] and a modified version of this protocol is presented in Subheading 3 of this chapter.In brief, SK-N-SH cells and derived clones including SH-SY5Y are plated at a density of 2 × 10 5 or 4 × 10 6 cells/60 mm dish in Eagle’s minimum … Weband SHSY5Y cells (neuroblastoma) in a 24-well format. For Gibco ™ Human Neural Stem Cells (hNSCs), 250 ng/well of mRNA was used in a 48-well format. Results are compared to plasmid DNA delivered with the leading DNA transfection reagent. GFP expression was analyzed 24 hours posttransfection. High transfection efficiency for neural cell models curly hair short mullet